An Introduction To Clean Rooms And Environmental Monitoring

A controlled environment is where the level of pollutants like aerosol particles

, dust, chemical vapors and airborne microbes is extremely low. The contamination level is fully controlled to provide a safe and health-friendly work environment. The rooms, requiring controlled environment, are classified according to the size and number of permitted particles per volume of air. Monitoring the entire atmosphere is the only way to obtain Crystal clarity. Humidity, temperature, pressurization and air flow rates need to be effectively controlled. Now industries are implementing up-to-date practices and innovative applications. They are using viable monitoring which includes, the testing of personnel, areas, air and surfaces to detect bacteria.

Following are the areas sampled in a clean room and ways to control contamination incurred due to them -

Surfaces This category includes walls, equipments and floors which are monitored and cleaned daily using contact plates. The Trypticase Soy Agar or TSA is a medium that is designed for bacteria and the Sabouraud Dextros Agar or SDA is designed for yeast and mold. The main equipments for surfaces include wringers and buckets, startup kit, detergents, disinfectants, dispensing bottles, sanitizers, lotions, sponges, shoe cleaners, mops, spray bottles, vacuum cleaners, squeegees and wipes.

Air The second most important field to control for viable counts, particle counts, humidity and temperature. High quality filters are used to monitor and control the viable, as well as non-viable particles. The most popular equipments used to maintain an appropriate level of air includes fume hoods, dust contaminant cabinets, laminar flow benches, showers, pass-through chambers, biological safety cabinet and ductless exhaust hoods.

Personnel They are the biggest contamination source in clean areas and carry millions of bacteria with them. A full spectrum of garments and accessories is available for more safety, protection against contamination and comfort. The type of garments includes lab coats, overalls, hoods, gowns, booties, masks and gloves. The fabric of these apparels is made from a patented spinning process to provide a barrier to microorganisms, dry particles and non-hazardous liquids.

A well-developed and well-implemented monitoring program is an effective way assuring the cleanliness of the industries. There are certain guidelines which should be followed for better results. All items such as watches, rings, keys and lighters should be stored in personal lockers separately. No eating, gum chewing and smoking should be permitted inside the work area and only approved garments should be used. No cosmetics should we worn such as eye shadow, lipstick, fingernail polish, perfumes, hair spray, after shaves, etc. Approved sanitizers and skin lotions should be kept in the washrooms to reduce skin flaking.

by: Sanny

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