Protein Expression Services A Critical Component In Clinical Trials

In the pharmaceutical industry, there has been a recent surge in the requirement of protein expression services

. Mammalian cell-related bioprocesses are the standard way to produce complex proteins like recombinant antibodies and there is a steady increase in the range of new biologics generated by mammalian cells. Besides this, the number of proteins to be quickly screened to find new candidates for clinical trials is also on the rise and this is being fulfilled by transient gene expression. This allows the manufacture of recombinant protein without the need to establish a stable cell line.

The transient gene expression method rapidly produces recombinant protein, making the pre-clinical material available to the drug screening process and enabling the speedy recruitment of volunteers for the study.

Why monoclonal antibody sequencing?

There are there critical reasons to sequence Monoclonal Antibodies:

While applying for a patent

For antibody recombination

Antibody humanization

When a monoclonal antibody must be patented, antibody sequencing helps differentiate the unique antibody from others. While sequencing the monoclonal antibody, the cDNA of the antibody can be separated from the hybridoma cell line, preventing its subcloning and expression in mammal cells. Antibody sequencing is also significant for an antibody humanization.

Why mammalian cells are preferred in protein expression services

Mammalian cells can carry out thorough post-translational modifications. They can secrete the right glycoproteins and have the required complex antennary oligosaccharides. Because of this, mammalian cells are the preferred choice for a variety of applications such as producing recombinant proteins, virus, gene therapy vectors and monoclonal antibody sequencing.Drug discovery projects need large scale protein expression services and mammalian cells are ideally suited for this.

Faster production

Transient protein expression services can help speed up the production and delivery of recombinant proteins and monoclonal antibodies sequencing. A growing protein drug market and rapid advance in proteomics requires large supplies of pure active proteins for producing protein drugs and in the development of drug assays. The production of large scale gene expression in mammalian cells is being recognized as the right method to produce the required amounts of recombinant proteins although the process is still being improved to increase productivity and quality.

This technology is preferred because it yields higher r-proteins through a serum-free medium. Another advantage is the high yield at reduced costs for a variety of applications over a short time-span. The proteins produced have high enzymatic activity.

Transient gene expression via transfection of mammalian cells is a popular method especially for variable quantities within a short time. HEK293 cells tend to involve high costs and low yields. The optimization of production criteria has helped to improve this. An increase in the yield of protein expression has been achieved through various improvements and process. The serum free medium altered by adding certain additives also increased the production of protein while stabilizing enzyme activity.

by: Paul Kerr

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