Reverse Phase and Normal Phase High Performance Liquid Chromatography

In the 1970s, reverse phase HPLC was developed

. The principle is opposite of normal phase system, where the stationary phase is packed non-polar material and the mobile phase is polar. Commonly used packing material in reverse phase columns are silica linked with carbon-18 (C18). There are other columns of more intermediate polarity, such as C8 and cyano.

Reverse Phase and Normal Phase High Performance Liquid Chromatography

By: Deevid Hiobson

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