The Use Of Capillary Electrophoresis For Purity, Heterogeneity And Genetic Analysis

Capillary Electrophoresis (CE) technology is employed in a series ofrelated separation

techniques that use narrow-bore fused-silica capillariesto separate a complex array of large and small molecules. Depending onthe types of capillary and buffers used, CE can be segmented into:

"Capillary Zone Electrophoresis (CZE)

"Capillary Gel Electrophoresis (CGE)

"Capillary Isoelectric Focusing (CIEF)

"Isotachophoresis (ITP)

"Electrokinetic Chromatography (EKC)

"MicellarElectrokinetic Capillary Chromatography (MECC)

"Capillary Electrochromatography (CEC)

"Non Aqueous Capillary Electrophoresis (NACE)

When capillary electrophoresis was introduced, it was seen as a revolutionary technique. Now,it"s fulfilling a role as a workhorse in analytical laboratories. It should be consideredfirst when dealing with highly polar, charged analytes and for genetic analysis. It has excelled in the rapid analysis ofions, and become predominant for impurity analysis of basic and chiral pharmaceuticals.

Chiral analysis

The construction of a chiral environment in CE is achieved by simply filling the capillary with a buffer containing achiral additive. Although many chiral selectors have been used, themost comprehensive and effective separation strategies have beenachieved with sulfated cyclodextrins.

Simplified Methods Development

CE"s chiral methods development strategy uses a family of highlysulfated cyclodextrins that have been developed and formulatedto functional specifications for enantiomer analysis, thus ensuring a reliable commercial supply of enantiomer selectors.This methods development strategy uses a single separation with , and highly sulfated cyclodextrins to resolve acidic,basic, neutral and zwitterionic pharmaceuticals. And by simplyreversing the sample introduction to the other side of the capillary, very rapid separations can be generated using only a 10 cm length.

Genetic Analysis

By simply introducing a polymer into the separation buffer, youcan rapidly create a physical gel toprovide a size-based size separation of nucleic acids. This approach""known as capillary gel electrophoresis has provided anautomated and quantitative replacement of gel electrophoresis foroligonucleotide quality control, RNA purity analysis, plasmid heterogeneity analysis, gene expression studies, and theanalysis of DNA/protein interactions.

Drug Screening

While the highly polar nature of basic pharmaceuticals makeschromatography complex, CE has the ability to excel atsuch separations. The robustnature of this tool and theruggedness of this particularapproach are appropriate forscreening protocols used in drugdiscovery and forensic toxicology aiding in purity analysis.

It is utilized in many different industries, including biological science, pharmaceutical, biotechnology, forensic science, and environmental safety. Its popularity can be attributed to its simplicity, reliability, speed and versatility, combined with low operating costs and minimal waste production.

Reference - https://www.beckmancoulter.com/wsrportal/bibliography?docname=BR-9515A1.pdf

by:Scott eric

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