subject: Reverse Phase and Normal Phase High Performance Liquid Chromatography [print this page] In the 1970s, reverse phase HPLC was developed. The principle is opposite of normal phase system, where the stationary phase is packed non-polar material and the mobile phase is polar. Commonly used packing material in reverse phase columns are silica linked with carbon-18 (C18). There are other columns of more intermediate polarity, such as C8 and cyano.
Reverse Phase and Normal Phase High Performance Liquid Chromatography
