subject: Learning to Use a Fluorescence Microscope [print this page] You should know that a florescent microscope is not like the common microscopes. It is specifically used to study organic or inorganic aspect by using florescence. Also, the object that is learnt is signed with a molecule that is called a fluorophore (a dye).
When the light of florescent is activated, the light that is used for illumination is apart from the florescent molecule that is much weaker. This is done through the filter of emission.
To use a fluorescence microscope, first of all, you should place the light switch on the microscope side that turns on the light. Then, turn the microscope on.
Do not forget to write the exact time you turn the light on. The light of florescent is mercury-based, and a time log should be kept for the illumination and light use.
You should also place the toggle switch on the right side of the microscope between the objectives and oculars. In this case, the switch is functional to control the shutter for the mercury light to the objective lens.
In addition, you should select the proper dye for your object. The most common dyes include I3 (for use with DTAF, CTC, and fluorscein), A (for use with f420 and DAPI), L3 (for use with fluorscein), and N21 (for use with rhodamine). Then, you can put the dye into the tray that is operated by the silver sliding knob.
Remember to choose the lens you will use. When you have finished, just turn off the light and do not forget to mark the time. Before turning on the light back, it is suggested for you to wait for 30 minutes to avoid the lamp from the explosion.
For the safety, you should replace the light based on the manual instructions from the manufacturer. Meanwhile, for maintenance, you should clean the lens of microscope with lens paper. If the lens is dirty, you can clean it by using a glass cleaner and cotton swap.
