subject: Henan Developed A New Rapid Detection Test Strip Of Chicken Diseases - China Stpp Sodium [print this page] Reporter learned from the Henan Academy of Agricultural Sciences, conducted by the institute, "French chicken infectious bursal disease research and application of probe test paper" project has achieved important results, the researchers successfully developed the world's first quick detection of infectious French capsule disease (IBD) virus, IBD virus rapid test strip, and obtained national patent.
China is a big country chickens, chicken 5 billion or more annually. French chicken infectious bursal disease of young chickens or broilers are the three major infectious diseases, IBD virus infection leading to immune defects induced by secondary infection or mixed infection of chickens, an immunosuppressive disease. The first occurred in China in 1979, has now spread throughout the country, its high incidence and mortality is usually 10% to 20%, sometimes up to 70%. Poultry industry has become a major disaster, serious impact on poultry industry healthy, efficient development. The traditional method of diagnosis and prevention of IBD in clinical diagnosis, virus isolation, identification, agar diffusion tests, these methods are not specific are not ideal, is not simple and quick, there are not easy to promote shortcomings. Therefore, to control and eradicate IBD need a specific, sensitive, simple, fast and suitable for a wide range of easy to promote the use of IBD antigen and antibody detection techniques.
According to reports, the project of similar studies at home and abroad in leading position, which uses genetic engineering to cloning and expression IBD VP2 or VP3 protein antigens, the establishment of secretion of the antigen monoclonal antibody hybridoma cell line pair, and then colloidal gold labeling technique and immunology research into new technologies, rapid test strip probe for the diagnosis of IBD and immune surveillance to provide a specific, sensitive, simple, fast, and easy to promote the use of new technology. The test strips can detect both antigen probe may also use the antibody inhibition assay, and therefore is immune diagnostic tool is generally applicable to teaching, research, clinical, production and quarantine authority, especially in the rural chicken farmers. In addition, the technology for the diagnosis of other infectious diseases and immune surveillance can provide a reference model.
According to the study the auspices of Vice President of Agricultural Sciences in Henan Province, Henan Province Key Laboratory of Animal Immunology Director of expression level of description, in the detection of IBD virus, without any additional equipment and reagents, simply insert the test strip sample, 1 to 5 minutes with visual to get the reliable results, the sensitivity of conventional agar diffusion test 64 times, can be quickly diagnosed IBD, IBD vaccine virus on the quick estimate or evaluation of content, and can inhibit the Rapid Determination of IBD in chickens home antibodies and immune antibodies for IBD susceptibility and immunity in chickens to monitor the effectiveness of assessment to guide the immune chickens IBD prevention and control. The results can be in all countries and regions, promote the use of chicken, is now in Henan, Hebei, Jiangsu, Shandong and other provinces to promote applications, covering more than 10,000 chickens in 4000 to reduce the economic losses of more than 1567 million. It is reported that the research project has received first prize for scientific and technological progress in Henan Province in 2003.
