subject: Henan successful production of influenza detection kit (Map) - Influenza - pharmaceutical industries [print this page] Henan successful production of influenza detection kit (Map) - Influenza - pharmaceutical industries
"Two hours! Just two hours, you can quickly test to determine whether patients infected with the influenza A H1N1 virus !"??( 6 pm) 4 pm, the reporter in Henan Engineering Research Center for Bio Molecular biological experiments room, see the Biotechnology Research Center of Henan Province, chief expert Wang Yunlong, his first move was good news out of two fingers!
5 11 at midnight, when reporters contact Wang Yunlong, the research team has battled hard with 10 days of the provincial research center leader biological test given in the latest standard operating hours or 3 hours, more rapid test kit is being tested, verification.
HC Pharmacy Industrial network with map
Less than 20 hours after continued efforts, Wang Yunlong team has broken one after another the other departments announced on May 3 to 5 hours to complete the pigs infected with influenza A H1N1 virus detection limit, minimum two hours to the results of a nationwide R & D-type H1N1 virus detection reagent "emergency in the Race", by running in the forefront.
6 pm when reporters on the scene were in the molecular biology laboratory, reagent preparation laboratory, and genetic engineering labs, see the center of molecular diagnostics expert Dr. Li Zhitao, Li Yulin, director of genetic engineering specialists, immunization diagnosis expert Susan Branch Professor and Dr. Liu Wanggen and other research team members are methodically working tension.
"If necessary, these reagents by a suitable means to free the quickest way to send to all levels of the new cities and counties in Henan Province Influenza Designated hospitals or designated health prevention and control institutions "?? Wang Yunlong answering questions from reporters, said.
5 7,'s telephone interview with Zhang, deputy director of Science and Technology Department of Henan Province on behalf of the people, asked his assessment of the results of the study, he said: "The very recognition! We should be prepared for." Reporter learned The Center is carrying out the advantage of rapid diagnosis of one of the units in the first study in China during the SARS SARS success of the first rapid diagnostic reagents and known access to national drug certification. Initially in the "swine flu" disease began before the release of the April 23, the center is sensitive to the team, has started to actively find related information do a lot of preparatory work.
2009 4 28 United States on public alpha (H1N1) influenza virus gene sequences, the central research team night analysis, design, multiple pairs of primers were optimized for three pairs of specific primers were synthesized good, after days and nights continuous research, experiment, verify, May 4 kit for success.
Wang Yunlong, said test kit for the successful development of efficient, thanks to the provincial party committee provincial government, the Zhengzhou municipal government attach great importance. Science and technology in our province and Zhengzhou city government department in charge of rapid response carefully arranged, the first time to support biotechnology research center in Henan Province of human infection with swine influenza start Epidemic Prevention and control strategies and key technology research, careful organization and scientific research early and rapid diagnostic reagents, the Provincial Science and Technology Department Director Zhao Chen, deputy director and director of special appointed Director of Social Development Centre in person to understand biological research and progress, to see scientists. Zhengzhou City Science and Technology Secretary Wang Jichang two research groups reported on the deployment of special arrangements to listen to related work, and to arrange and social development, Deputy Secretary in charge Director of the first line in person, on-site to solve some research on the problems encountered. Research team worked day and night through the research and experiments have been used center technology platform for rapid diagnosis of diseases and in molecular biology, molecular hybridization, gene amplification and other aspects of the advantages of accumulated technology, successfully developed such a rapid, specific, sensitive Influenza A (H1N1) influenza virus detection kit.
The reagent has three distinctive features :
An advanced technology. The design of the project has the following six technical advantages: the use of advanced triple PCR technique; using one-step rapid test; by UNG pollution prevention system; using component separation and release, to ensure high specificity; used throughout every step of the internal control system detects reaction; with nucleoprotein NP, neuraminidase (NA), hemagglutinin (HA) 3 gene in multiple confirmed to detect were more detailed understanding of the characteristics of the sample.
2, speed, and can be detected within 2 hours. Provincial Life Centre with high efficiency, sophisticated nucleic acid extraction technology that can be completed in half an hour fast, high-yield target template extraction process, compared with the traditional method to triple its output, while in the process of triple PCR amplification technology to operate double time and effort to reduce 2 / 3.
3, results are reliable. The project uses the amplified fragments of different sizes of three pairs of primers, PCR technology using triple swine influenza A (H1N1) genes were detected, while adding a whole system of reference to track, because the operator can avoid a result of incorrect operation miscarriage of justice or the kit itself, the result of problems caused by false positives, and can ensure sensitivity of detection.
The purpose of the project using a simple and quick detection method for qualitative strip test, has wide adaptability. Equipment only general PCR, can be early and rapid diagnosis, with the maneuverability is strong, using the broad features. In the current "swine flu" continues to spread the grim situation, in time, rapid diagnosis is extremely important. Currently, the successful development of the project, is actively involved in science and technology of Henan Province "swine flu" prevention and control of action and important stage, we will have far-reaching in Henan Province's social health impact.
