subject: Biodetection Technologies - 6th Edition --- Aarkstore Enterprise [print this page] The 6th edition in our Detection Technology series is an internationally recognized publication for experts in detection & identification of biological threats. This publication will explore the latest R&D developments including:
Point-of-care/clinical applications for pathogen/virus/threat detection
and identification
Technological challenges for rapid/early/specific sensitive detection
Role of nanotechnology and system miniaturization
Bioinformatics for biodefense
Reducing false positives vs. detector sensitivity
Field-deployable devices:
portability/compatibility/reliability/scalability
Reagentless vs. reagent biodetection systems
Non-PCR vs. PCR based detection techniques
Advances in microarray and sequencing technologies
Issues of sensitivity/testing/evaluation/validation
Table of Contents :
CHAPTER 1
Multiplex Real-Time PCR Assay Design for Pathogen Detection, Quantification and Speciation
Robert S. Tebbs, PhD, Staff Scientist, Applied Biosystems*
A multiplex real-time PCR assay was designed to identify 3 species of Vibrio and an internal positive control using a four-dye configuration. Multiple strains were sequenced to identify target sites. Several individual assays within the multiplex contain more than one primer or probe due to strain to strain polymorphisms. Vibrio cholerae, V. parahaemolyticus, and V. vulnificus were detected either individually or in mixtures at 25 genomic copies. The Vibrio multiplex assay showed 100% specificity to all targets analyzed and no detection of an exclusion panel that included nearest neighbors. Each assay in the multiplex exhibited 100 10% efficiency over a 5 log range. Multiplex real-time PCR can simplify pathogen detection and reduce costs since 3 species can be analyzed in a single reaction tube. *In collaboration with: P.M.Brzoska, S.Bit, M.R.Furtado, and O.V.Petrauskene
CHAPTER 2
Multiplexed Diagnostic Assays for Viral Pathogens of Cattle and Swine
Max V. Rasmussen, PhD, Biomedical Scientist, Biosciences Defense Division, Lawrence Livermore National Laboratory
Diagnostic tests for animal pathogens support a healthy livestock industry and reliable food supply. Lawrence Livermore National Laboratory developed multiplexed assays that simultaneously discriminate nucleic acid sequences of foreign and endemic viral pathogens affecting cattle and swine in a high-throughput format. The porcine-disease assay detects foot-and-mouth disease, vesicular stomatitis, swine vesicular disease, vesicular exanthema of swine and porcine reproductive and respiratory syndrome. The bovine-disease assay detects foot-and-mouth disease, malignant catarrhal fever, rinderpest, bovine viral diarrhea, bluetongue, bovine papular stomatitis, psuedocowpox, infectious bovine rhinotracheitis, vesicular stomatitis and bovine herpes mammalitis. Ongoing DHS-funded research includes enhancing sensitivity to FMDV, supporting the USDA-APHIS assay validation process and development of a FMDV sub-typing microarray.
