subject: development of visual DNA chip technique for detection of HIV-1 infection [print this page] Probes were bond covalently on the surface of DNA chip which were hybridized to the products of final step. At the end of this step and washing, DNA chip was subjected to specific anti deoxygenize anti bodies which were mixed by alkaline phosphatase enzyme in the presence of BCIP/NBT substrate. Forming the blue color represents the presence of virus. The advantages to this technique are: Specialty, high sensitivity, short time, easy and doable in most of the labs and also cheaper in comparison with similar techniques.
development of visual DNA chip technique for detection of HIV-1 infection
