High Performance Liquid Chromatography

High Performance Liquid Chromatography also referred to as HPLC is described as an

analytical tool that quantifies, divides and identifies objects within a sample. It is often used in the biochemistry and analytical chemistry industries. It is a system that carries the sample by utilizing a combination of solvents to the stationary phase. This is where the departure of compounds takes place. Then a detector gathers the departed compounds and signals are delivered to the integrator to create a graphic visual.

High Performance Liquid Chromatography includes the following components:

Auto Sampler (or Injector Port)

Analytical samples are presented through this particular component. All samples must be manually injected with the correct HPLC syringes. The auto sample allows a person to load each sample into the HPLC system. At pre-set conditions, the system automatically chooses the proper sample to inject.

Detector

The detector is a unit that recognizes the departed compounds within the sample. There are an assortment of detectors that utilizes various modes of detection, such as refractive index, fluorescence, mass spectroscopy and ultra-violet rays.

Mobile Phase

This consists of a combination of solvents that are used to disperse the samples throughout the entire system. All of the solvents must be miscible in the solution. Otherwise, the immiscible solvents will trigger pressure build-up within the High Performance Liquid

Chromatography system. The analysis length and the separation of compounds are affected by the ratios of every solvent component within the mobile phase.

Chromatography

When samples are dispersed through the column, all of the compounds within the sample will interact at the same time. It interacts with the mobile and stationary phase in order to output a different elution time for every compound. The purpose of each analysis is to divide the peak of interest from all the other remaining compounds.

Integrator

An integrator turns the signals from detector into what is known as chromatograms (a visual output). At one time, they came from older computer systems that utilized paper charts.

Today, integrators are generated through computer systems.

Stationary Phase

The stationary phase is often referred to as the column. This portion of the HPLC system is considered to be the heart of separation. It derives from securely packed material within a stainless steel column. High pressure is needed in order to disperse the solvents throughout the system. This is often referred to as high pressure liquid.

Solvent Delivery Unit (or Pump)

The purpose of this component is to send the samples and mobile phase throughout the HPLC system at a steady flow frequency. Often times, an HPLC pump is set in order to function at a steady flow rate.

Contact Buck Scientific at 800.562.5566 to learn more about our Infrared Accessories, High Performance Liquid, Spectroscopy Accessories and Supplies.

by: Ban ronaldo

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