Biodetection Technologies 5th Edition - Technological Response To Biological Threats ---- Aarkstore

With an emphasis on rapid detection and low-cost identification

, this publication provides solutions to the challenges presented by the evolving pattern of complex biological threat agents of various origins.

Completely updated with the latest information, including narratives, charts, graphs and question & answers, this essential reference tool addresses the following important topics:

- PCR and Non-PCR Based Detection

- Reagent and Reagentless Based Detection

- Point-of-Care Toxin & Pathogen Detection

- Utilizing Bacteriophage for Biological Threat Response

- Real-World Sample Preparation for Toxin & Pathogen Detection

Table of Contents :

Chapter 1

The Neccesity for Improving Biodetection Technologies

Robert Hooks, Deputy Assistant Secretary for WMD and Biodefense, U.S. Department of Homeland Security

In the biodefense area, DHS efforts focus on providing leadership by coordinating with other Federal partners, state/local/tribal jurisdictions, the private sector and the international community. In addition to developing technologies for Biowatch or NBIS, which are two DHS program areas, improved technology is equally important for our partners and is critical to improving the nations capabilities in the biodefense arena.

Chapter 2

SAFE: Sequencing for Avian Flu Epidemic

Niveen Mulholland, PhD, Senior Scientist, MRI-NCR, Midwest Research Institute*

We have developed a system for detecting mutations that would give rise to potential pandemic-causing influenza strains. The system, SAFE: Sequencing for Avian Flu Epidemic, first uses real time RT-PCR to detect H5, the highly pathogenic avian influenza most likely to cause a pandemic. We next use pyrosequencing to detect codon changes encoding amino acids known to define human versus avian influenza signatures. The SAFE real time RT-PCR assay specifically detects H5 in a multiplex reaction designed to detect a region of the Matrix gene common to all Influenza A subtypes. The H5 primer/probe set is specific; it does not cross react with other Influenza A subtypes tested. The M primer/probe set serves as an internal control, detecting all subtypes tested. Pyrosequencing assays were developed to screen, at the nucleotide level, for 52 amino acids changes defined by Chen et al (2006) to be avian- or human- specific. A library has been built to screen the sequence data generated and properly identify the strain in question as a potential threat. This surveillance system described here will allow the global community to monitor for H5N1 and for mutations that will render the virus more infective and virulent to humans. *In collaboration with: N.Waybright, E.Petrangelo, P.Lowary & J.Bogan

Chapter 3

Next Generation Automated Multi-Target Detection Platform for Closer to Source Diagnostics

Todd Ritter, CEO of Applied Science & Technology, Idaho Technology Inc.

Idaho Technology has developed a highly multiplexed detection system capable of concurrently identifying and genetically discriminating dozens of viruses and bacteria. The syringe-loaded system utilizes a flexible plastic pouch combining automated sample preparation, reverse transcription for RNA viruses, and two-stage nested multiplex PCR performing 120 discreet analyses simultaneously. Capable of processing a variety of sample types, the small, lightweight diagnostic system represents a next generation in automated detection systems.

Chapter 4

Rapid Multiplexed Nucleic Acid and Antibody Based Sensor for Biothreat Detection

Michael R. Meyer, Director of Laboratories, ICx Biosystems

RapidPlex is a fully automated, triggered confirmation system designed for detection and identification of bacteria, virus, and toxin threats in 10 minutes. The RapidPlex system provides simultaneous, multiplexed detection of protein and DNA/RNA markers through parallel antibody and nucleic acid-based assays. Antibody-based detection utilizes multiplexed sandwich assays on spectrally encoded microspheres followed by high resolution imaging of individual beads for detection of spores/cells captured on the bead surfaces as well as detection of toxins and viruses coating the bead surfaces. The nucleic acid-based process includes automated cell lysis and purification, followed by multiplexed DNA/RNA amplification and detection of the DNA amplicons on spectrally encoded microspheres.

Chapter 5

Multiplex Detection of Biothreat Agents by Fluidic Force Discrimination

Gary W. Long, PhD, Vice President and Senior Scientist, Tetracore, Inc.

Fluidic Force discrimination (FFD) has recently been applied to the detection of Biothreat agents. In this talk, we will describe the multiplex detection of 3 or more agents by an immunoassay developed for FFD, and demonstrate that sensitivity of detection is similar or greater than that obtained in lateral flow devices and by ELISA. We will also describe improvements to the sensitivity of detection of nucleic acids using this technology.

Chapter 6

Use of CANARY for Rapid, Automated Collection and Analysis of Bioaerosol Samples

Thomas Hazel, PhD, Vice President Research, Innovative Biosensors, Inc.

CANARY is a cell-based technology that enables rapid identification of bacterial, viral, and toxin targets in liquid or aerosol samples. In this presentation we describe the testing and validation of our new BioFlash instrumentation, designed to provide detect-to-protect capability for environmental monitoring and building security applications. This platform enables automated collection, detection, and simultaneous identification of up to 21 target aerosol agents with increased speed and sensitivity.

Chapter 7

SAW RFID Biosensors for Ubiquitous Molecular Recognition

William D. Hunt, PhD, Professor, School of Electrical and Computer Engineering, Georgia Institute of Technology*

Research by Hunts group at Georgia Tech has demonstrated that an acoustic wave biosensor with a biolayer coating can detect analytes such as Bascillus spores, cocaine, TNT/RDX in the gaseous phase. A new embodiment of this approach involves a SAW RFID/biosensor, which can passively sense and report on molecules in its environment. The SAW RFID/biosensor system involves a main interrogation unit transmitting an RF signal to a passive SAW structure located a short distance away. The device combined with the antenna re-transmits a reflected acoustic wave, which has been perturbed by the on-chip molecular recognition events. *In collaboration with: P.J.Edmonson, D.D.Stubbs, Zen Sensing LLC; D.Bertieri, Penn United Technologies Inc.

For more information, please visit :

http://www.aarkstore.com/reports/Biodetection-Technologies-5th-Edition-Technological-Response-to-Biological-Threats-1747.html

by: Aarkstore Enterprise

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