Tips For Choosing The Best Secondary Antibodies For Your Scientific Needs

Secondary antibodies are highly important for scientists and researchers in their experiments and advanced research

. As a scientist, in order to get the best result out of your experiment, you should always choose the right secondary antibody. So, how do you know which secondary antibody best meets the requirements of your research? Well, mentioned below are some tips that will help you select the right secondary antibody.

First of all you should know the host species of the primary antibody. Why? Well, because the secondary antibody is directed against the species of the primary antibody. For instance if you use a primary antibody raised in goat, you will require an anti-goat secondary antibody raised in a species which is other than goat. You should always remember the fact that secondary antibodies work in specific applications. Now, for any given application several similar secondary antibodies may be available. Also, primary antibodies help you determine antigen whereas secondary antibodies are meant to detect a primary antibody.

As a scientist, you should also know about the sub-class the primary antibody belongs to and find out a suitable marker for secondary antibody. When you select a secondary antibody, you should select the suitable secondary antibody for recognition of the primary antibody like IgG, IgM, IgE, IgD or IgA. Now you would think what is the difference between these? Well, the significant difference lies in their heavy chains. These are denominated with Greek letters which are the basis for the antibodies names, gamma, mu, epsilon, delta and alpha. Now, each of the antibodies has two light chains which also must be considered for some applications.

It is highly recommended that you choose an expert antibodies supplier to meet your scientific needs and requirements. For this, you need to do some research. You can easily get the best suppliers online. However, you should consider some factors while choosing a secondary antibodies supplier. Know the suppliers reputation in the market, experience and technical expertise. You should also find out at what prices the supplier offers the antibodies and what is the quality of those antibodies. Many experienced suppliers offer the best technical support for choosing both primary antibodies and secondary antibodies.

You can also use antibody comparison sites for secondary antibody search. The comparison websites are a reliable resource that helps scientists and researchers to compare cots and the application of multiple vendors in one place. You can easily search both the gene as well as the protein names of your target to get the best results. Choose a supplier which offers you custom antibody development services at affordable prices. Their team of experienced scientists will help you select the best antibody in quick time.

by: Biorbyt

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