There is lot of points to be remembered while using a pipette
. First of all daily wipe the pipette with 70% ethanol so that there won't be dirt on the pipette. Now check whether the volume is in specified range. While holding the pipette the "grippy finger rest" should rest on the index finger. To get the maximum accuracy the pipette, tip and liquid should be kept at the same temperature. Its always better to use high quality tips which is made from contamination-free polypropylene for the pipette, so that the tip will ensure maximum accuracy. The metrological characteristics of the tips will change after each time of use so it should be cleaned after use. Its better to pre rinse the tip three to four times with the liquid to be used. It will definitely increase the accuracy especially when using positive displacement tips. Don't turn the pipette on its side when there is liquid in the tip, liquid must go to the interior and contaminate it. Always try to store the pipettes on stands in upright position when not in use. If used daily it is recommended to check the calibration in three month interval. Always follow the instructions of recalibration.