Activity Of Enzyme Induction Technology Can Improve The Content Of Active Ingredients Of Medicinal

In recent years, with the progress of science and technology and medical research a higher level

, from the natural plant extract active ingredient pharmaceutical research and develop new drugs has become one of the hot. Activity of enzyme induction technology is a plant biotechnology engineering of new technologies, it from the perspective of active enzyme induction start to promote the bio-synthesis of a large number of the active ingredient in vivo. Zhejiang University, Biomedical Engineering and Instrument Science, Associate Dean Professor Tian Jingkui said that it will apply the new biotechnology, the field of traditional medicine, the activity of enzyme induction technology, will become the promotion of pharmaceutical manufacturing and plant extracts industry booster.

Extraction of active ingredients face difficulties

In plants there are some very strong pharmacological effects of active ingredients, such as the yew roots, stems and leaves contain a kind of taxol can inhibit tumor of tricyclic diterpenoids. However, these active ingredients in plants is very low levels, becoming a bottleneck to limit its extraction and purification. For example, in the Mandia Taxus paclitaxel content of the foliage of only about three ten thousandths, separation and purification very difficult.

"Bio-pharmaceutical industry after years of research, the accumulation and use of some industrial production methods is a relatively mature, but they are very cumbersome because of its steps, making the high cost of pharmaceutical products." TIAN Jing-kui said. Bio-pharmaceutical technology companies, research institutes, have never stopped a strong pharmacological effects of these active ingredients extraction techniques, with a view to simplify the extraction process as much as possible to reduce production costs.

TIAN Jing-kui, at present the method in the research focused on extraction technology and process, there are also plants by improving the cultivation of medicinal plants, methods to increase its active ingredients. For example, in the extraction of artemisinin, through taking polyploid induction and related cultivation methods to develop new crop strains of artemisinin. The technology can overcome the artemisinin content of crops under natural conditions is low, the quality problems of instability and improve the artemisinin content of crops and shorten the growth cycle, so as to achieve increased levels of raw materials, lower production cost.

Inventive activity of enzyme induction techniques

"Compared with the previous studies described, the active enzyme induction technology is different from the extraction process, as well as the transformation process, but also different from the improvement of medicinal plants, plant cultivation methods of innovation, but rather another way, through the activity of enzyme induction in vivo biological activity of a large number of synthesis ingredients. "TIAN Jing-kui said in conjunction with their work.

The technology includes three studies. First, the natural product-depth study of the mechanism of secondary metabolites. Such as through the yew, mulberry leaf, ginkgo leaf, the active ingredient artemisinin biosynthesis of key enzymes in the research, to clarify the relevant biological activity of natural products synthesis mechanism. Second, the orientation of biological active substances inducing. Such as through the optimization of induction conditions, to carry out taxol, artemisinin, ginkgo flavonoids, camptothecin-induced activity of enzymes targeted experiments, to improve the yield of active ingredients. 3 is a significant efficacy, the structure induced by novel compounds directed. Activity of enzyme induction in the course of the study, will get some new structure of secondary metabolites. Such as to induce from the ginkgo leaf out of sulfur-containing aromatic compounds, there is a good antioxidant and antibacterial effects; from mulberry leaves Induced Sangpi contained only in the Diels-Alder type adducts in biology, there are anti-oxidation, anti-inflammatory effects.

TIAN Jing-kui, etc. with the relevant units through long-term scientific research, by optimizing the activity of enzymes induced by a variety of technical conditions so that a variety of medicinal plants have been a very low content of active ingredients to be a more substantial increase. To Ginkgo biloba, for example, through the activity of enzyme induction technology, an increase of flavonoid content. Among them, an increase of 22% quercetin and isorhamnetin increased by 17%, kaempferol increased by 13%. Pilot-scale production of studies have shown that the application of the technology content of total flavonoids can increase by 30% ~ 60%. This technology of Ginkgo biloba for drug processing and production provides a good technological base, greatly improving production efficiency, reduce production costs. Again, through the activity of enzyme induction, Mandia of taxol content in Taxus in a steady increase in more than 70% of artemisinin content of Artemisia annua a steady increase in more than 100%.

Industrial development of promising

TIAN Jing-kui said that as a new biological engineering technology, active enzyme induction as compared with traditional methods have more advantages, for the comprehensive development of medicinal plants and utilization of great significance for the development of the industrialization of medicinal plants has a very important facilitating role.

The technology features and advantages of the maximum extraction rate. Of raw materials, such as Taxol yew trees, is the world's rare and endangered species, in our country as his home a protected plant. Although there were four kinds in our country, and a variety of yew, but the overall view [FS: PAGE], the plant's resources are very scarce, and slow growth. Coupled with the bark of Taxus paclitaxel were very low (about 0.02%), complicated chemical synthesis steps, each 10 tons of bark in order to extract 1 kg of paclitaxel, resulting in shortage of the drug is very expensive. Through active enzyme induction technology, the bark of the yew taxol content increased 70%, so that it can greatly reduce the exploitation and utilization of tree species.

For Artemisia annua and artemisinin, the traditional method of cultivation is mainly through different measures to improve the production of Artemisia annua and artemisinin content. However, the above method due to temperature, light and other conditions, coupled with the promotion of medicinal plant cultivation techniques require extensive technical training, etc., the cost is large and the result is not satisfactory. The Extraction of artemisinin-induced enzyme activity does not require a lot of technology promotion, does not require input of plant cultivation, only a technical induced by pharmaceutical companies to complete.

Ginkgo biloba extract flavonoids from the traditional approach is referred to by alcohol macroporous resin purification, although to be effective in extract of Ginkgo biloba in the pharmaceutical active ingredients flavonoids and terpene lactones, flavonoid content, but still relatively low, only at 24% or so. The activity of enzyme induction technology can be used for the Ginkgo biloba extract flavonoid compounds provide a new way.

In addition, the simple process, low energy consumption is also the activity of enzyme induction technology advantages. As the activity of enzyme induction technology is only for enzyme induction activity of medicinal plants, and will not result in extraction of the complex and processes on the complex, pharmaceutical companies can only invest a small amount of equipment, technical process is very simple.

However, TIAN Jing-kui said, this technology there are some shortcomings, such as only the roots, stems, branches, leaves, skin type medicines good effect, while for fruit, flowers and seeds, the effects in general medicine. Currently, they are also being increased research efforts with a view to improve the quality of these medicines on the breakthrough.

Activity Of Enzyme Induction Technology Can Improve The Content Of Active Ingredients Of Medicinal

By: wuwu

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