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development of visual DNA chip technique for detection of HIV-1 infection

Probes were bond covalently on the surface of DNA chip which were hybridized to the products of final step

. At the end of this step and washing, DNA chip was subjected to specific anti deoxygenize anti bodies which were mixed by alkaline phosphatase enzyme in the presence of BCIP/NBT substrate. Forming the blue color represents the presence of virus. The advantages to this technique are: Specialty, high sensitivity, short time, easy and doable in most of the labs and also cheaper in comparison with similar techniques.

development of visual DNA chip technique for detection of HIV-1 infection

By: danial
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development of visual DNA chip technique for detection of HIV-1 infection